Hight –efficient production of recombinant mGM-CSF
2008
Hochschulschrift
Zugriff:
96
The plant cells are a Eukaryotic cell system whit can produce biologically active protein. During the last 20 years, the recombinant DNA technology has enabled the production of many important biopharmaceuticals and of genetically enhanced herbicide pest resistant plants that has a significant positive impact on health care and on agriculture. The Plant cell express system has been investigated for their attractive merits: 1、Simple medium economic cost. 2、Similar post-translational modifications to that of human. 3、low level of risk for contamination by mammalian virus, for blood –borne pathogens, and for bacterial toxins. 4、Its yield promotes its industrial production of high pure protein. However, there are no cost effective fermentation technique development because of the slow cells growth rate and low production efficiency. The purpose of this study is to develop a cultivate strategy and develop a bioreactor to proliferate rice cell for producing recombinant proteins. In suspension cell culture, we compare artificial sugar starvation and natural sugar starvation in a flask scale culture to produce recombinant proteins with cell growth and production. The result showed natural sugar starvation had better productivity of 5.0 mg / L. In immobilization cell culture, we designed three different immobilization methods in our study. The first design was fixed 6*6*1.5 cm of polyurethane foams in shaking flask with productivity of 0.06 mg / L. The second design was using two-way bubble circular reactor (TBCR) to immobilize cell. We used two kinds carriers one polyurethane foams and the other loofa sponge. We find the viability of cell on the PU better the loofa carriers, but higher concentration of immobilized cells on the loofa sponge.
Titel: |
Hight –efficient production of recombinant mGM-CSF
|
---|---|
Autor/in / Beteiligte Person: | Young Chen Chen ; 陳勇臣 |
Link: | |
Veröffentlichung: | 2008 |
Medientyp: | Hochschulschrift |
Sonstiges: |
|