Effect of Sesame Oil against Thioacetamide-induced Hepatic Injury in Rats

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Introduction: Thioacetamide (TAA) is used to induce many acute hepatic injuries, such as fulminant hepatic failure (FHF) in animal models which relate to oxidative stress injuries. Many studies show that sesame oil (SO) protects liver through decrease oxidative stress and inflammation in acute animal models. Objective: To investigate the effect of SO on TAA-induced acute hepatic injury. Design: TAA (100 mg/kg; i.p) was used to induce acute hepatic injury. SO (1, 2, 4, and 8 ml/kg; orally) was administrated on different time points (0, 6, and 12h) after TAA injection. The serum and liver tissue samples were collected to analyze liver injuries on hepatic aspartate aminotransferase (AST), alanine aminotransferase (ALT), lipid peroxidation (LPO) level and inflammatory regulators such as myeloperoxidase (MPO), interleukin-6 (IL-6) and interleukin-10 (IL-10). Results: There was no significant difference between AST and ALT by using single dose of SO treatment. Double dose of SO treatments (0 and 12 h) with in 24 h also showed no significant difference on TAA-induced acute hepatic injury. However, the triple dose of SO treatments (0, 6, and 12 h) showed a significant decreased on AST and ALT with 4 ml/kg sesame oil, and the MDA level in liver also showed significant decreased. Also MPO activity was decreased after sesame oil treatment. Conclusion: SO attenuated TAA-induced acute hepatic injury significantly by using triple SO oral treatments. And the protection of SO was related with inhibition of MPO activity, furthermore, improved LPO injury.