Effect of zingerone on the anti-oxidation and anti-inflammatory in rat kidney epithelial cells
2010
Hochschulschrift
Zugriff:
98
Ginger is a common spice used in popular cuisine and has been widely applied in research of diseases due to its rich ingredients. The purpose of this research is to probe into the antioxidation activity and anti-inflammatory effect of zingerone (ZO) of NRK 52E cells (Normal rat kidney epithelial cells). The experiment design covers two parts: the first test is “In vitro” in which ZO of different concentrations (i.e. 1, 5, 7.5, 10 mM) are used to analyze DPPH activity, SOD, FIC and reducing power. The results show that ZO demonstrates good effects in removing DPPH activity and reducing power. Moreover, the ZO 10 mM has the highest removal rate of 19% on SOD activity; however, the ZO demonstrates no FIC ability. The second test is the “Ex-vivo” in which t-BHP 2 mM is used to induce NRK 52E oxidative injuries and ZO of different concentrations (i.e. 1, 5, 7.5, 10 mM) are added and cultured at 37°C to evaluate the antioxidation ability after 15, 30, and 60 minutes. With antioxidation analysis indicators including MTT assay and TBARs and GSH, the results show that ZO causes no effect on the survival rate of cells. As ZO increases in concentration, the production of TBARs is significantly reduced and GSH expression is enhanced. In addition, the IL-1β 5 ng is used to induce NRK 52E inflammation and ZO of different concentrations (i.e. 1, 5, 7.5, 10 mM) are added and cultured at 37°C to evaluate the anti-inflammatory ability after 20 hours. Measured analysis of the survival rate, NO, iNOS, COX-2, PGE2, IκB, p-IκB and NF-κB. The results showed that ZO 1, 5, 7.5, 10 (mM) compared with the control group, the survival rate is no change, in IL-1β + ZO this group 4 there are cells to reduce the generation of NO, iNOS, PGE2 and COX-2 performance (p < 0.05). On the other hand, p-IκB and NF-κB performance has also been decline. The study, ZO inhibits inflammation caused by IL-1β induced NRK 52E to achieve anti-inflammatory effect. At the same time, it is able to inhibit oxidative injuries caused by t-BHP induced NRK 52E thus protecting kidney cells.
Titel: |
Effect of zingerone on the anti-oxidation and anti-inflammatory in rat kidney epithelial cells
|
---|---|
Autor/in / Beteiligte Person: | Lin, Pei-ying ; 林佩瑩 |
Link: | |
Veröffentlichung: | 2010 |
Medientyp: | Hochschulschrift |
Sonstiges: |
|