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The p38 mitogen-activated protein kinase (p38MAPK) plays a key role in larval settlement of the barnacle Amphibalanus amphitrite. To study the signaling pathway associated with p38MAPK during larval settlement, we sought to identify the upstream kinase of p38MAPK. Three MKKs (MKK3, MKK4 and MKK7) and three MAPKs (p38MAPK, ERK and JNK) in A. amphitrite were cloned and recombinantly expressed in E. coli. Through kinase assays, we found that MKK3, but not MKK4 or MKK7, phosphorylated p38MAPK. Furthermore, MKK3 activity was specific to p38MAPK, as it did not phosphorylate ERK or JNK. To further investigate the functional relationship between MKK3 and p38MAPK in vivo, we studied the localization of phospho-MKK3 (pMKK3) and MKK3 by immunostaining. Consistent with the patterns of p38MAPK and phospho-p38MAPK (pp38MAPK), pMKK3 and MKK3 mainly localized to the antennules of the cyprids. Western blot analysis revealed that pMKK3 levels, like pp38MAPK levels, were elevated at cyprid stage, compared to nauplii and juvenile stages. Moreover, pMKK3 levels increased after treatment with adult barnacle crude extracts, suggesting that MKK3 might mediate the stimulatory effects of adult barnacle extracts on the p38MAPK pathway. © 2013 Zhang et al.

Titel:	MKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK
Link:	View this record from OAIster (Volltext)
Veröffentlichung:	2013
Medientyp:	Elektronische Ressource
Schlagwort:	Index Terms: mitogen activated protein kinase kinase 3 mitogen activated protein kinase kinase 4 mitogen activated protein kinase kinase 7 mitogen activated protein kinase p38 stress activated protein kinase Amphibalanus amphitrite barnacle cypris (larva) enzyme activity immunohistochemistry in vivo study larval development nauplius (larva) nucleotide sequence protein localization protein phosphorylation Western blotting Extracellular Signal-Regulated MAP Kinases JNK Mitogen-Activated Protein Kinases Larva MAP Kinase Kinase 3 MAP Kinase Kinase 4 MAP Kinase Kinase 7 p38 Mitogen-Activated Protein Kinases Thoracica Article
Sonstiges:	Nachgewiesen in: OAIster Added Details: Zhang, Gen ; He, Li-Sheng ; Wong, Yue Him ; Qian, Pei-Yuan Document Type: Electronic Resource Availability: Open access content. Open access content ; This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ; Archived with thanks to PLoS ONE Note: Zhang G, He L-S, Wong YH, Qian P-Y (2013) MKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK. PLoS ONE 8: e69510. doi:10.1371/journal.pone.0069510. ; English Contributing Source: KING ABDULLAH UNIV OF SCI & TECH ; From OAIster®, provided by the OCLC Cooperative.

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MKK3 Was Involved in Larval Settlement of the Barnacle Amphibalanus amphitrite through Activating the Kinase Activity of p38MAPK