Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR
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Elektronische Ressource
The heterotrophic dinoXagellate PWesteria piscicida was detected in Ace Lake in the Vestfold Hills, eastern Antarctica by using real-time PCR based on 18S rDNA sequences. Antarctic water samples collected in 2004 were tested by species-speciWc real-time PCR assays for the identiWcation of P. piscicida and P. shumwayae. Positive results were shown with P. piscicida-speciWc real-time PCR, and PCR products were examined by sequence analysis for conWrmation. A phylogenetic tree made from partial 18S rDNA sequences showed that the Antarctic clone clustered with P. piscicida. This result suggests that P. piscicida is present in the extreme conditions of an Antarctic saline lake which has not contained Wsh for thousands of years.
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Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR
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Medientyp: | Elektronische Ressource |
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