Proof of principle study on a novel setup of the biological detection chain : combined use of a bioaerosol wetted wall sampler and a microfluidic sensor to perform trigger to analyse
2022
Online
Elektronische Ressource
NLDA Masterthesis / MTPS
The capability to timely and reliably detect the presence of airborne threat agents is one of the challenges the Netherlands Ministry of Defence (NLD MoD) is facing. Whereas technology is not yet able to provide a detect-to-warn capability, a detect-to-treat capability is more feasible. This capability involves a chain of bio-agent sampling, triggering and identifying aimed at providing bio-detection signals allowing timely medical treatment. However, usefulness of current solutions is limited because the interplay between samplers and trigger detectors is falling short. This results in large numbers of irrelevant samples offered to the identification equipment, making the capability highly time-consuming and labor-intensive. Furthermore current triggering technology results in too high false positive rate. This research aimed to combine the sampling and triggering steps in optimal interplay. A novel set up of a part of the detection chain is proposed and evaluated using a Wetted Wall Cyclone (WWC)-sampler combined with a microfluidic Electrochemical Impedance Spectroscopy (EIS)-sensor used as a trigger to analyse, to improve the detection chain in both the response time and logistical footprint. The research question focuses on the extent to distinguish airborne biological threat agents from non-biological particles using a WWC-sampler and an EIS-sensor. Both techniques are tested taking into account various settings, environmental factors and the interoperability of the devices. The results show the possibility to perform batch-wise high volume sampling followed by a technique to screen the collected samples on the presence of bacteria. The sensitivity to discriminate bacteria from non-biological particles is such the collected sample can be analysed by e.g. Lateral Flow Assay (LFA) near the detection threshold. The collected sample itself is in addition useable for confirmed and unambiguous identification.
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Proof of principle study on a novel setup of the biological detection chain : combined use of a bioaerosol wetted wall sampler and a microfluidic sensor to perform trigger to analyse
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Veröffentlichung: | 2022 |
Medientyp: | Elektronische Ressource |
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