Early Assessment of Atherosclerotic Lesions and Vulnerable Plaques in vivo by Targeting Apoptotic Macrophages with AV Nanobubbles
2022
Online
Elektronische Ressource
Xiaoju Ma,1,2,* Jia Wang,1,* Zhelong Li,1 Xueying Zhou,3 Xiao Liang,1 Junyan Wang,4 Yunyou Duan,1 Ping Zhao1 1Department of Ultrasonic Medicine, Tang Du Hospital, Air Force Medical University, Xi’an, 710038, People’s Republic of China; 2Ultrasonic Department, Lin Tong Rehabilitation and Convalescent Center, Lintong, 710600, People’s Republic of China; 3Ultrasonic Department, Air Force Hospital of Central Theater, Datong, 037006, People’s Republic of China; 4Department of Nuclear Medicine, Tang Du Hospital, Air Force Medical University, Xi’an, 710038, People’s Republic of China*These authors contributed equally to this workCorrespondence: Ping Zhao; Yunyou Duan, Email zhaoping812@163.com; duanyy@fmmu.edu.cnBackground: The early detection of atherosclerotic lesions is particularly important for risk prediction of acute cardiovascular events. Macrophages apoptosis was significantly associated with the degree of AS lesions and especially contributed to plaque vulnerability. In this research, we mainly sought to explore the feasibility of a home-made AV-nanobubbles (NBAV) for visualization of apoptotic macrophages and assessment of atherosclerosis (AS) lesions by contrast-enhanced ultrasound (CEUS) imaging.Methods: NBAV were prepared by “Optimized Thin-Film Hydration” and “Biotin-Avidin-Biotin” methods. Then, the characterization and echogenicity of NBAV were measured and analyzed in vitro. The targeting ability of NBAV to ox-LDL–induced apoptotic macrophages was observed by laser scanning confocal microscope. The ApoE−/− mice mode fed with high fat diet were observed by high-frequency ultrasound, microanatomy and oil red O staining. CEUS imaging in vivo was performed on AS plaques with NBAV and NBCtrl injection through the tail vein in turn in ApoE−/− mice. After CEUS imaging, the plaques were confirmed and analyzed by his
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Early Assessment of Atherosclerotic Lesions and Vulnerable Plaques in vivo by Targeting Apoptotic Macrophages with AV Nanobubbles
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Veröffentlichung: | 2022 |
Medientyp: | Elektronische Ressource |
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