Regulated Proteolysis: The Secrets of Lon : Cellular Roles and Stress-dependent Regulation of the Lon Protease
2023
Online
Elektronische Ressource
Proteolysis is crucial in cells to maintain a functional proteome. It is required for removal of damaged and unfolded proteins during protein quality control, and serves as a mechanism to regulate protein levels through regulated proteolysis. The latter targets specific proteins under certain conditions to rapidly adjust the levels of these proteins. Thereby, activity of the targeted proteins is reduced or even eliminated. Many cellular processes like cell cycle progression, differentiation and stress response/adaptation depend on proteolytic removal of regulatory proteins via proteolysis. One protease with crucial roles in both protein quality control and regulated proteolysis is called Lon and is found in many species across all domains of life. Extensive research has revealed many aspects of proteolysis by Lon and identified several Lon substrates. The dimorphic bacterium Caulobacter crescentus is a model organisms to study proteolysis and Lon as the two cell types, a flagellated swarmer cell and a stalked sessile cell, and the transition between them heavily depends on proteolysis. However, certain details, like recognition of substrates and its regulation are largely unknown. Here I focus on regulated proteolysis by Lon in C. crescentus, specifically on novel substrates, their recognition, and the regulation of Lon. Study I: Using quantitative proteomics of wildtype and mutant C. crescentus strains we identified potential substrates of Lon. Out of these, we focused on the stalk biogenesis regulator StaR and the flagellar hook length determination factor FliK. Both proteins are developmental regulators, whose protein levels oscillate during the cell cycle. Our experiments showed that turnover by Lon is required to maintain these oscillations and disruption thereof results in deregulation of the stalk and the flagellum. Study II: We used proteolytically inactive Lon to co-purify interactors of Lon and identify them by mass spectrometry. Thereby, we found an uncha
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Regulated Proteolysis: The Secrets of Lon : Cellular Roles and Stress-dependent Regulation of the Lon Protease
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Veröffentlichung: | 2023 |
Medientyp: | Elektronische Ressource |
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