Performance evaluation of a MIP for the MISPE-LC determination of p-[18F]MPPF and a potential metabolite in human plasma
Elsevier, 2020
Online
academicJournal
Zugriff:
Within the family of serotonin (5-HT) receptors, the 5-HT1A subtype is particularly interesting as it may be involved in various physiological processes or psychological disorders. The p-[18F]MPPF, a highly selec- tive 5-HT1A antagonist, is used for in vivo studies in human or animal by means of positron emission tomography (PET) [1].In order to selectively extract p-[18 F]MPPF and its main metabolites from plasma, molecularly imprinted polymer (MIP) was prepared against these compounds by using the p-MPPF as template. For the control of the selectivity, non-imprinted polymer (NIP) was also synthesized without template. The MIP sorbent, packed in disposable extraction cartridges (DECs), was then evaluated as molecularly imprinted solid- phase extraction (MISPE) prior to the LC determination. The conditions of extraction were evaluated in order to obtain the highest selective retention of the p-[18F]MPPF and its metabolites on this MIP. The MIP selectivity was exploited in the loading and washing steps by adjusting the pH of plasma samples at a suitable value and by selecting mixtures for the washing step to limit the contribution of non- specific interactions. Other important parameters involved in the conditioning and elution steps were also studied. Finally, a pre-validation was carried out with optimal extraction conditions to demonstrate the performance of this MISPE-LC method as a generic method in the context of evaluation of new MISPE for p-[18F]MPPF and its potential for metabolites extraction from human plasma.
FP6 STRP516984 MI-lab- on-chip
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Performance evaluation of a MIP for the MISPE-LC determination of p-[18F]MPPF and a potential metabolite in human plasma
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Autor/in / Beteiligte Person: | Lecomte, Frederic ; Aerts, Joël ; Plenevaux, Alain ; Defraiteur, Caroline ; Chappuis-Hugon, Florence ; Rozet, Eric ; CHIAP, Patrice ; Luxen, André ; Pichon, Valérie ; Hubert, Philippe ; Hubert, Cédric ; CIRM - Centre Interdisciplinaire de Recherche sur le Médicament - ULiège |
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Veröffentlichung: | Elsevier, 2020 |
Medientyp: | academicJournal |
DOI: | 10.1016/j.jpba.2019.113015 |
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