The role of inflammatory conditions on DBBM resorption- an investigation in a murine calvaria model
Morressier, 2017
Online
unknown
Objectives: The behavior of deproteinized bovine bone mineral (DBBM) under inflammatory condition remains unclear. The aim of the study was to investigate the possible resorption of DBBM under local inflammatory conditions in vivo using the calvarial osteolysis modelMethods: In Thirty adult BALB/c mice, DBBM was implanted into the space between the elevated periosteum/soft tissue and the calvaria. Inflammation was induced either by LPS or polyethylene particles (Ceridust) mixed with DBBM. Three treatments modalities were randomly applied (n = 10 each): i) DBBM alone (Control) ii) DBBM + polyethylene particles (Ceridust) and iii) DBBM + LPS. Mice were euthanized on day fourteen and each calvarium was subjected to histological and u00b5CT analysis. Primary outcome parameter was the size distribution of the DBBM particles. Secondary outcome parameter was the surface erosion of the calvaria bone. Results: Histological and u00b5CT analysis revealed that the size distribution and the overall volume in the defect the DBBM particles was similar between DBBM alone and the combinations with LPS or polyethylene particles. Moreover, histological evaluation showed no signs of erosions of DBBM particles under inflammatory conditions. u00b5CT analysis further revealed that LPS and the polyethylene particles, but not the DBBM alone, caused severe remodeling of the calvaria bone as indicated by cortical porosity, the latter was confirmed by histological analysis. Conclusions: These outcomes suggest that local host calvaria bone but not the DBBM particles undergo severe remodeling under inflammatory condition in a mouse augmentation model.
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The role of inflammatory conditions on DBBM resorption- an investigation in a murine calvaria model
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Veröffentlichung: | Morressier, 2017 |
Medientyp: | unknown |
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