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- Nachgewiesen in: USPTO Patent Applications
- Sprachen: English
- Document Number: 20110252508
- Publication Date: October 13, 2011
- Appl. No: 13/140322
- Application Filed: December 10, 2009
- Assignees: BASF Plant Science GmbH (Ludwigshafen, DE)
- Claim: 1.-133. (canceled)
- Claim: 134. A method for enhancing a yield-related trait and/or abiotic stress tolerance in a plant relative to a corresponding control plant, comprising modulating expression in a plant cell, plant, or part thereof a nucleic acid selected from the group consisting of: (a) a nucleic acid encoding a CRSP33-like polypeptide comprising one or more of the following motifs: (i) Motif I: YPPPPPFYRLYK (SEQ ID NO: 35) or a motif having at least 90% sequence identity to Motif I; (ii) Motif II: QGVRQLYPKGP (SEQ ID NO: 36) or a motif having at least 90% sequence identity to Motif II; (iii) Motif III: LNRELQLHILELADVLVERPSQYARRVE (SEQ ID NO: 37) or a motif having at least 90% sequence identity to Motif III; and (iv) Motif IV: IFKNLHHLLNSLRPHQARAT (SEQ ID NO: 38) or a motif having at least 90% sequence identity to Motif IV; (b) a nucleic acid encoding an MCB polypeptide; (c) a nucleic acid encoding an SRT2 polypeptide; (d) a nucleic acid encoding a YRP2 polypeptide or an orthologue or paralogue thereof; (e) a nucleic acid encoding a YRP3 polypeptide or an orthologue or paralogue thereof; (f) a nucleic acid encoding a YRP4 polypeptide or an orthologue or paralogue thereof; and (g) a nucleic acid encoding an SPX-RING polypeptide.
- Claim: 135. The method of claim 134, wherein the modulated expression is effected by introducing and expressing the nucleic acid in a plant or plant cell.
- Claim: 136. The method of claim 134, wherein (a) the nucleic acid encoding a CRSP33-like polypeptide comprises (i) a polynucleotide encoding any one of the proteins listed in Table A1, a portion of said polynucleotide, or a nucleic acid capable of hybridising with said polynucleotide; (ii) a polynucleotide encoding a polypeptide having at least 90% sequence identity to SEQ ID NO: 4; or (iii) a polynucleotide encoding an orthologue or paralogue of any of the proteins given in Table A1; (b) the nucleic acid encoding an MCB polypeptide comprises (i) a polynucleotide encoding an MCB polypeptide comprising one or more motifs having at least 50% sequence identity to any one or more of the following motifs: [table included] wherein amino acids between brackets represent alternative amino acids at the position; (ii) a polynucleotide encoding any one of the proteins listed in Table A2, a portion of said polynucleotide, or a nucleic acid capable of hybridising with said polynucleotide; or (iii) a polynucleotide encoding an orthologue or paralogue of any of the proteins given in Table A2; (c) the nucleic acid encoding an SRT2 polypeptide comprises (i) a polynucleotide encoding an amino acid domain having at least 50% overall sequence identity to any one or more of the amino acid domains set forth in Table C1; (ii) a polynucleotide encoding any one of the proteins listed in Table A3, a portion of said polynucleotide, or a nucleic acid capable of hybridising with said polynucleotide; or (iii) a polynucleotide encoding an orthologue or paralogue of any of the proteins set forth in Table A3; (d) the nucleic acid encoding a YRP2 polypeptide or an orthologue or paralogue thereof comprises (i) a polynucleotide encoding any one of the proteins listed in Table A4, a portion of said polynucleotide, or a nucleic acid capable of hybridising with said polynucleotide; or (ii) a polynucleotide encoding an orthologue or paralogue of any of the proteins given in Table A4; (e) the nucleic acid encoding a YRP3 polypeptide or an orthologue or paralogue thereof comprises; (i) a polynucleotide encoding any one of the proteins listed in Table A5, a portion of said polynucleotide, or a nucleic acid capable of hybridising with said polynucleotide; or (ii) a polynucleotide encoding an orthologue or paralogue of any of the proteins given in Table A5; (f) the nucleic acid encoding a YRP4 polypeptide or an orthologue or paralogue thereof comprises (i) a polynucleotide encoding any one of the proteins listed in Table A6, a portion of said polynucleotide, or a nucleic acid capable of hybridising with said polynucleotide; or (ii) a polynucleotide encoding an orthologue or paralogue of any of the proteins given in Table A6; or (g) the nucleic acid encoding an SPX-RING polypeptide comprises (i) a polynucleotide encoding a motif having at least 50% overall sequence identity to any one or more of: Motifs 1-1 to Motifs 1-35 (SEQ ID NO: 340 to 374); Motifs 2-1 to Motifs 2-35 (SEQ ID NO: 375 to 409); and Motifs 3-1 to Motifs 3-35 (SEQ ID NO: 410 to 444); (ii) a polynucleotide encoding any one of the proteins listed in Table A7, a portion of said polynucleotide, or a nucleic acid capable of hybridising with said polynucleotide; or (iii) a polynucleotide encoding an orthologue or paralogue of any of the proteins given in Table A7.
- Claim: 137. The method of claim 134, wherein the enhanced yield-related trait is increased yield, increased seed yield, and/or increased biomass relative to a corresponding control plant.
- Claim: 138. The method of claim 134, wherein the enhanced yield-related trait is obtained under non-stress conditions.
- Claim: 139. The method of claim 134, wherein the nucleic acid is operably linked to a constitutive promoter, a GOS2 promoter, or a GOS2 promoter from rice.
- Claim: 140. The method of claim 134, wherein (a) the nucleic acid encoding a CRSP33-like polypeptide is of plant origin, from a dicotyledonous plant, from the family Solanaceae, or from Lycopersicum esculentum; (b) the nucleic acid encoding an MCB polypeptide is of plant origin, from a dicotyledonous plant, from the family Brassicaceae, from the genus Arabidopsis, or from Arabidopsis thaliana; (c) the nucleic acid encoding a SRT2 polypeptide is of plant origin, from a dicotyledonous plant, from the family Brassicaceae, from the genus Arabidopsis, or from Arabidopsis thaliana; (d) the nucleic acid encoding a YRP2 polypeptide is from Solanum lycopersicon; (e) the nucleic acid encoding a YRP3 polypeptide is from Physcomitrella patens; (f) the nucleic acid encoding a YRP4 polypeptide is from Triticum aestivum; or (g) the nucleic acid encoding an SPX-RING polypeptide is of plant origin, from a dicotyledonous plant, from the family Brassicaceae, from the genus Arabidopsis, or from Arabidopsis thaliana.
- Claim: 141. The method of claim 134, wherein expression of the nucleic acid is increased.
- Claim: 142. A method for producing seeds comprising (a) obtaining the plant produced by the method of claim 134; and (b) harvesting seeds from said plant.
- Claim: 143. A construct comprising: (i) a nucleic acid; (ii) one or more control sequences capable of driving expression of said nucleic acid; and optionally (iii) a transcription termination sequence, wherein said nucleic acid is selected from the group consisting of: (a) a nucleic acid encoding a CRSP33-like polypeptide comprising one or more of the following motifs: (i) Motif I: YPPPPPFYRLYK (SEQ ID NO: 35) or a motif having at least 90% sequence identity to Motif I; (ii) Motif II: QGVRQLYPKGP (SEQ ID NO: 36) or a motif having at least 90% sequence identity to Motif II; (iii) Motif III: LNRELQLHILELADVLVERPSQYARRVE (SEQ ID NO: 37) or a motif having at least 90% sequence identity to Motif III; and (iv) Motif IV: IFKNLHHLLNSLRPHQARAT (SEQ ID NO: 38) or a motif having at least 90% sequence identity to Motif IV; (b) a nucleic acid encoding an MCB polypeptide; (c) a nucleic acid encoding an MCB polypeptide which comprises one or more motifs having at least 50% sequence identity to any one or more of the following motifs: [table included] wherein amino acids between brackets represent alternative amino acids at the position; (d) a nucleic acid encoding an SRT2 polypeptide; (e) a nucleic acid encoding an SRT2 polypeptide comprising a protein domain having at least 50% overall sequence identity to any one or more of the amino acid domains set forth in Table C1; (f) a nucleic acid encoding a YRP2 polypeptide or an orthologue or paralogue thereof; (g) a nucleic acid encoding a YRP2 polypeptide or an orthologue or paralogue thereof, wherein the nucleic acid encodes any one of the proteins listed in Table A4, or is a portion of such a nucleic acid, or a nucleic acid capable of hybridising with such a nucleic acid; (h) a nucleic acid encoding a YRP3 polypeptide or an orthologue or paralogue thereof; (i) a nucleic acid encoding a YRP3 polypeptide or an orthologue or paralogue thereof wherein the nucleic acid encodes any one of the proteins listed in Table A5 or is a portion of such a nucleic acid, or a nucleic acid capable of hybridising with such a nucleic acid; (j) a nucleic acid encoding a YRP4 polypeptide or an orthologue or paralogue thereof; (k) a nucleic acid encoding a YRP4 polypeptide or an orthologue or paralogue thereof wherein the nucleic acid encodes any one of the proteins listed in Table A6 or is a portion of such a nucleic acid, or a nucleic acid capable of hybridising with such a nucleic acid; (l) a nucleic acid encoding an SPX-RING polypeptide; and (m) a nucleic acid encoding an SPX-RING polypeptide comprising a motif having at least 50% overall sequence identity to any one or more of: (i) Motifs 1-1 to Motifs 1-35 (SEQ ID NO: 340 to 374); (ii) Motifs 2-1 to Motifs 2-35 (SEQ ID NO: 375 to 409); and (iii) Motifs 3-1 to Motifs 3-35 (SEQ ID NO: 410 to 444).
- Claim: 144. The construct of claim 143, wherein one of the control sequences is a constitutive promoter, a GOS2 promoter, or a GOS2 promoter from rice.
- Claim: 145. A plant, plant part, or plant cell transformed with the construct of claim 143.
- Claim: 146. A plant, plant part, or plant cell obtained by the method of claim 134, wherein the plant, plant part, or plant cell comprises the nucleic acid.
- Claim: 147. A transgenic plant having increased yield and/or increased abiotic stress tolerance relative to a corresponding control plant, or a transgenic plant cell derived from said transgenic plant, wherein the increased yield and/or abiotic stress tolerance results from modulated expression of a nucleic acid selected from the group consisting of (a) a nucleic acid encoding a CRSP33-like polypeptide comprising one or more of the following motifs: (i) Motif I: YPPPPPFYRLYK (SEQ ID NO: 35) or a motif having at least 90% sequence identity to Motif I; (ii) Motif II: QGVRQLYPKGP (SEQ ID NO: 36) or a motif having at least 90% sequence identity to Motif II; (iii) Motif III: LNRELQLHILELADVLVERPSQYARRVE (SEQ ID NO: 37) or a motif having at least 90% sequence identity to Motif III; and (iv) Motif IV: IFKNLHHLLNSLRPHQARAT (SEQ ID NO: 38) or a motif having at least 90% sequence identity to Motif IV; (b) a nucleic acid encoding an MCB polypeptide; (c) a nucleic acid encoding an MCB polypeptide which comprises one or more motifs having at least 50% sequence identity to any one or more of the following motifs: [table included] wherein amino acids between brackets represent alternative amino acids at the position; (d) a nucleic acid encoding an SRT2 polypeptide; (e) a nucleic acid encoding an SRT2 polypeptide comprising a protein domain having at least 50% overall sequence identity to any one or more of the amino acid domains set forth in Table C1; (f) a nucleic acid encoding a YRP2 polypeptide; (g) a nucleic acid encoding a YRP3 polypeptide; (h) a nucleic acid encoding a YRP4 polypeptide; (i) a nucleic acid encoding an SPX-RING polypeptide; and (j) a nucleic acid encoding an SPX-RING polypeptide comprising a motif having at least 50% overall sequence identity to any one or more of: (i) Motifs 1-1 to Motifs 1-35 (SEQ ID NO: 340 to 374); (ii) Motifs 2-1 to Motifs 2-35 (SEQ ID NO: 375 to 409); and (iii) Motifs 3-1 to Motifs 3-35 (SEQ ID NO: 410 to 444).
- Claim: 148. The method of claim 135, further comprising cultivating the plant or plant cell under conditions promoting plant growth and development and/or under abiotic stress conditions.
- Claim: 149. The plant of claim 145, or a transgenic plant cell derived from the plant, wherein the plant is a crop plant, a monocot, a cereal, rice, maize, wheat, barley, millet, rye, triticale, sorghum, emmer, spelt, secale, einkorn, teff, milo, or oats.
- Claim: 150. Harvestable parts of the plant of claim 149.
- Claim: 151. The harvestable parts of claim 150, wherein said harvestable parts are seeds.
- Claim: 152. Products derived from the plant of claim 149 and/or from harvestable parts of said plant.
- Claim: 153. A method for producing a transgenic plant with enhanced abiotic stress tolerance and/or an enhanced yield-related trait relative to a corresponding control plant, comprising transforming a plant with the construct of claim 143.
- Current U.S. Class: 800/290
- Current International Class: 01; 01; 12; 01; 12
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