Einzeltreffer — DigiBib

A separation module operates to fractionate or separate an analyte into fractions according to pI, i.e., pI bands, utilizing capillary isoelectric focusing (“CIEF”) within a first microchannel. The fractions are stacked to form plugs, the number of which is determined by a number of parallel second microchannels integrally connected to the first microchannel, into which the fractions are directed according to the buffer characteristics found in each of the individual microchannels. Within the microchannels the plugs are separated into proteins according to a different chemical property, i.e., “m/z,” utilizing capillary electrophoresis (“CE”).

Titel:	System and Method For The Separation of Analytes
Autor/in / Beteiligte Person:	Schneider, Thomas Wayne ; Baraniuk, James N.
Link:	View record in USPTO Patent Applications (Volltext)
Veröffentlichung:	2012
Medientyp:	Patent
Sonstiges:	Nachgewiesen in: USPTO Patent Applications Sprachen: English Document Number: 20120080316 Publication Date: April 5, 2012 Appl. No: 13/323813 Application Filed: December 12, 2011 Claim: 1. A system for separating a sample comprising: means for introducing a sample into a first microchannel formed in a module; means for separating the sample into a plurality of sub-samples based on varying isoelectric points of the plurality of sub-samples; means for causing a first of the plurality of sub-samples to enter a first of a plurality of second parallel microchannels integrally connected to the first microchannel; means for separating the first of the plurality of sub-samples into a plurality of protein components according to electrophoresis; means for applying opposite charges to either end of the first microchannel to facilitate separating the sample into the plurality of sub-samples; means for applying a first charge to the non-intersecting end of a third microchannel integrally connected to the first microchannel and applying a second opposite charge to the non-intersecting end of the first of the plurality of second microchannels to facilitate the first of the plurality of sub-samples entering the first of the plurality of second microchannels and further separating according to electrophoresis; means for aligning a first detector with the first microchannel in order to detect the separation of the sample into the plurality of sub-samples based on varying isoelectric points of the plurality of sub-samples within the first microchannel; means for aligning a second detector with the non-intersecting end of the first of the plurality of second microchannels; and means for detecting the plurality of protein components for the first of the plurality of sub-samples. Claim: 2. The system according to claim 1, wherein the means for detecting comprises UV-Visible (“UV-Vis”) and photodiode (“PDA”) arrays. Claim: 3. The system according to claim 2, wherein the UV-Vis and PDA arrays continuously monitor the separation of the sample into the plurality of sub-samples. Claim: 4. The system according to claim 1, wherein each of the plurality of sub-samples contains at least one known component and at least one unknown component. Claim: 5. The system according to claim 4, wherein the at least one known component is an internal standard and the at least one unknown component is a protein component. Claim: 6. The system according to claim 5, wherein the protein component is at least one of an amino acid, a peptide, a polypeptide or a protein. Claim: 7. The system according to claim 1, wherein the second detector is a mass spectrometer that produces a spectrum indicative of each of the plurality of protein components. Claim: 8. The system according to claim 1, wherein the means for detecting the plurality of protein components comprises a mass spectrometer for producing a spectrum indicative of each of the plurality of protein components. Claim: 9. The system according to claim 1, wherein the means for aligning the first detector with the first microchannel comprises an X-Y translator. Claim: 10. A system for separating a sample comprising: injection adapters that introduce a sample into a first microchannel formed in a module; a first microchannel of the module that separates the sample into a plurality of sub-samples based on varying isoelectric points of the plurality of sub-samples; at least one second microchannel that separates a first of the plurality of sub-samples into a plurality of protein components according to electrophoresis; electrodes that apply opposite charges to either end of the first microchannel to facilitate separating the sample into the plurality of sub-samples; a translator that aligns a first detector with the first microchannel to detect the separation of the sample into the plurality of sub-samples based on varying isoelectric points of the plurality of sub-samples within the first microchannel, and aligns a second detector with one microchannel of the at least one second microchannel. Claim: 11. The system according to claim 10, wherein the first detector comprises UV-Visible (“UV-Vis”) and photodiode (“PDA”) arrays. Claim: 12. The system according to claim 11, wherein the UV-Vis and PDA arrays continuously monitor the separation of the sample into the plurality of sub-samples. Claim: 13. The system according to claim 10, wherein each of the plurality of sub-samples contains at least one known component and at least one unknown component. Claim: 15. The system according to claim 13, wherein the at least one known component is an internal standard and the at least one unknown component is a protein component. Claim: 16. The system according to claim 15, wherein the protein component is at least one of an amino acid, a peptide, a polypeptide or a protein. Claim: 17. The system according to claim 10, wherein the second detector is a mass spectrometer that produces a spectrum indicative of each of the plurality of protein components. Claim: 18. A system for separating a sample comprising: a module containing microchannels; injection adapters that introduce a sample into a first microchannel of the microchannels; electrodes that apply opposite charges to either end of the first microchannel to facilitate separating the sample into the plurality of sub-samples; an electrode that applies a charge to at least one second microchannel of the microchannels to separate a first of the plurality of sub-samples into a plurality of protein components; a translator that aligns a first detector with the first microchannel to detect the separation of the sample into the plurality of sub-samples, and aligns a second detector with one microchannel of the at least one second microchannel. Claim: 19. The system according to claim 18, wherein the first detector comprises UV-Visible (“UV-Vis”) and photodiode (“PDA”) arrays. Claim: 20. The system according to claim 19, wherein the UV-Visible (“UV-Vis”) and photodiode (“PDA”) arrays continuously monitor the separation of the sample into the plurality of sub-samples. Current U.S. Class: 204/603 Current International Class: 01; 25

Klicken Sie ein Format an und speichern Sie dann die Daten oder geben Sie eine Empfänger-Adresse ein und lassen Sie sich per Email zusenden.

BibTeX Citavi, JabRef, u.a.
(Literaturverwaltung)

PDF kein Volltext!
(Merkzettel, Notizen)

RIS Endnote, Citavi u.a.
(Literaturverwaltung)

MODS
(XML zur Weiterverarbeitung)

oder

Wählen Sie das für Sie passende Zitationsformat und kopieren Sie es dann in die Zwischenablage, lassen es sich per Mail zusenden oder speichern es als PDF-Datei.

Gewünschter Zitations-Stil:

oder

Bitte prüfen Sie, ob die Zitation formal korrekt ist, bevor Sie sie in einer Arbeit verwenden. Benutzen Sie gegebenenfalls den "Exportieren"-Dialog, wenn Sie ein Literaturverwaltungsprogramm verwenden und die Zitat-Angaben selbst formatieren wollen.

System and Method For The Separation of Analytes