Einzeltreffer — DigiBib

This disclosure provides an improved system for culturing human pluripotent stem cells. Traditionally, pluripotent stem cells are cultured on a layer of feeder cells (such as mouse embryonic fibroblasts) to prevent them from differentiating. In the system described here, the role of feeder cells is replaced by components added to the culture environment that support rapid proliferation without differentiation. Effective features are a suitable support structure for the cells, and an effective medium that can be added fresh to the culture without being preconditioned by another cell type. Culturing human embryonic stem cells in fresh medium according to this invention causes the cells to expand surprisingly rapidly, while retaining the ability to differentiate into cells representing all three embryonic germ layers. This new culture system allows for bulk proliferation of pPS cells for commercial production of important products for use in drug screening and human therapy.

Titel:	PRIMATE PLURIPOTENT STEM CELL EXPANSION WITHOUT FEEDER CELLS AND IN THE PRESENCE OF FGF AND MATRIGEL OR ENGELBRETH-HOLM-SWARM TUMOR CELL PREAPARATION
Link:	View record in USPTO Patent Applications (Volltext)
Veröffentlichung:	2014
Medientyp:	Patent
Sonstiges:	Nachgewiesen in: USPTO Patent Applications Sprachen: English Document Number: 20140302600 Publication Date: October 9, 2014 Appl. No: 12/710078 Application Filed: February 22, 2010 Claim: 1-20. (canceled) Claim: 21. A system for expanding primate pluripotent stem cells in vitro without feeder cells comprising a nutrient media and a soluble preparation from Engelbreth-Holm-Swarm tumor cells. Claim: 22. The system of claim 21, wherein the nutrient media is conditioned by a feeder cell. Claim: 23. The system of claim 21, wherein the nutrient media is a fresh media that is not conditioned by a feeder cell. Claim: 24. The system of claim 21, wherein the nutrient media comprises a fibroblast growth factor. Claim: 25. A system for expanding a cell expressing the markers stage specific antigen 4 (SSEA4), Tra-1-60 and Tra-1-81 in vitro without feeder cells comprising a nutrient media and a soluble preparation from Engelbreth-Holm-Swarm tumor cells. Claim: 26. A method of culturing primate pluripotent stem cells in vitro without feeder cells comprising culturing the primate pluripotent stem cells on a soluble preparation from Engelbreth-Holm-swarm tumor cells and contacting the cells with a nutrient media. Claim: 27. The method of claim 26, wherein the nutrient media is conditioned by a feeder cell. Claim: 28. The method of claim 26, wherein the nutrient media is a fresh media that is not conditioned by a feeder cell. Claim: 29. The method of claim 26, wherein the nutrient media comprises a fibroblast growth factor. Claim: 30. A method of culturing a cell expressing the markers stage specific antigen 3 (SSEA3), stage specific antigen 4 (SSEA4), Tra-1-60 and Tra-1-81 in vitro without feeder cells comprising culturing the cell expressing the markers, stage specific antigen 4 (SSEA4), Tra-1-60 and Tra-1-81 on a soluble preparation from Engelbreth-Holm-Swarm tumor cells and contacting the cell with a nutrient media. Current U.S. Class: 435/363 Current International Class: 12

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PRIMATE PLURIPOTENT STEM CELL EXPANSION WITHOUT FEEDER CELLS AND IN THE PRESENCE OF FGF AND MATRIGEL OR ENGELBRETH-HOLM-SWARM TUMOR CELL PREAPARATION