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- Nachgewiesen in: USPTO Patent Applications
- Sprachen: English
- Document Number: 20230056618
- Publication Date: February 23, 2023
- Appl. No: 17/875549
- Application Filed: July 28, 2022
- Claim: 1. A modified ABCB9 gene encoding a protein conferring resistance to CMV in a plant of the Cucurbitaceae, in which the protein is expressed, characterized in that the gene comprises a nucleotide sequence of a) a nucleotide sequence which encodes a protein comprising SEQ ID NO: 4; b) a nucleotide sequence comprising SEQ ID NO: 3; c) a nucleotide sequence encoding a protein derived by substitution, deletion and/or addition of one or more amino acids of the protein comprising SEQ ID NO: 4; d) a nucleotide sequence that encodes a protein comprising an amino acid sequence, which is at least 85% identical to SEQ ID NO: 4; e) a nucleotide sequence which is at least 85% identical to SEQ ID NO: 3; or f) a nucleotide sequence according to c) or d) wherein the protein comprises a methionine (M) on position 428 of SEQ ID NO: 4, or on a position corresponding thereto.
- Claim: 2. A protein conferring resistance to CMV in a plant of the Cucurbitaceae, in which the protein is expressed and which protein is encoded by the gene of claim 1.
- Claim: 3. A modified EF1-α gene encoding a protein conferring resistance to CMV in a cucumber plant, in which the protein is expressed, characterized in that the gene comprises a nucleotide sequence of a) a nucleotide sequence which encodes a protein comprising SEQ ID NO: 8; b) a nucleotide sequence comprising SEQ ID NO: 7; c) a nucleotide sequence encoding a protein derived by substitution, deletion and/or addition of one or more amino acids of the protein comprising SEQ ID NO: 8, d) a nucleotide sequence that encodes a protein comprising an amino acid sequence, which is at least 95% identical to SEQ ID NO: 8; e) a nucleotide sequence which is at least 95% identical to SEQ ID NO: 7; or f) a nucleotide sequence according to c) or d) wherein the protein comprises a Glycine (G) on position 372 of SEQ ID NO: 8, or on a position corresponding thereto.
- Claim: 4. A protein conferring resistance to CMV in a plant of the Cucurbitaceae, in which the protein is expressed and which protein is encoded by the gene of claim 3.
- Claim: 5. A plant of the Cucurbitaceae comprising the modified ABCB9 gene of claim 1.
- Claim: 6. The plant of claim 5 comprising the modified ABCB9 gene homozygously, wherein the plant is resistant to CMV.
- Claim: 7. The plant of claim 5, further comprising a modified EF1-α gene encoding a protein conferring resistance to CMV in a cucumber plant, in which the protein is expressed, characterized in that the gene comprises a nucleotide sequence of a) a nucleotide sequence which encodes a protein comprising SEQ ID NO: 8; b) a nucleotide sequence comprising SEQ ID NO: 7; c) a nucleotide sequence encoding a protein derived by substitution, deletion and/or addition of one or more amino acids of the protein comprising SEQ ID NO: 8, d) a nucleotide sequence that encodes a protein comprising an amino acid sequence, which is at least 95% identical to SEQ ID NO: 8; e) a nucleotide sequence which is at least 95% identical to SEQ ID NO: 7; or f) a nucleotide sequence according to c) or d) wherein the protein comprises a Glycine (G) on position 372 of SEQ ID NO: 8, or on a position corresponding thereto.
- Claim: 8. The plant of claim 5, comprising the modified EF1-α gene, homozygously.
- Claim: 9. A part of the plant of claim 5, which plant part comprises the modified ABCB9 gene and optionally a modified EF1-α gene encoding a protein conferring resistance to CMV in a cucumber plant, in which the protein is expressed, characterized in that the gene comprises a nucleotide sequence selected from a) a nucleotide sequence which encodes a protein comprising SEQ ID NO: 8; b) a nucleotide sequence comprising SEQ ID NO: 7; c) a nucleotide sequence encoding a protein derived by substitution, deletion and/or addition of one or more amino acids of the protein comprising SEQ ID NO: 8, d) a nucleotide sequence that encodes a protein comprising an amino acid sequence, which is at least 95% identical to SEQ ID NO: 8; e) a nucleotide sequence which is at least 95% identical to SEQ ID NO: 7; or f) a nucleotide sequence according to c) or d) wherein the protein comprises a Glycine (G) on position 372 of SEQ ID NO: 8, or on a position corresponding thereto.
- Claim: 10. A seed comprising the modified ABCB9 gene of claim 1, and optionally a modified EF1-α gene encoding a protein conferring resistance to CMV in a cucumber plant, in which the protein is expressed, characterized in that the gene comprises a nucleotide sequence of a) a nucleotide sequence which encodes a protein comprising SEQ ID NO: 8; b) a nucleotide sequence comprising SEQ ID NO: 7; c) a nucleotide sequence encoding a protein derived by substitution, deletion and/or addition of one or more amino acids of the protein comprising SEQ ID NO: 8, d) a nucleotide sequence that encodes a protein comprising an amino acid sequence, which is at least 95% identical to SEQ ID NO: 8; e) a nucleotide sequence which is at least 95% identical to SEQ ID NO: 7; or f) a nucleotide sequence according to c) or d) wherein the protein comprises a Glycine (G) on position 372 of SEQ ID NO: 8, or on a position corresponding thereto.
- Claim: 11. A tissue culture of the plant of claim 5, which tissue culture comprises the modified ABCB9 gene, and optionally a modified EF1-α gene encoding a protein conferring resistance to CMV in a cucumber plant, in which the protein is expressed, characterized in that the gene comprises a nucleotide sequence of a) a nucleotide sequence which encodes a protein comprising SEQ ID NO: 8; b) a nucleotide sequence comprising SEQ ID NO: 7; c) a nucleotide sequence encoding a protein derived by substitution, deletion and/or addition of one or more amino acids of the protein comprising SEQ ID NO: 8, d) a nucleotide sequence that encodes a protein comprising an amino acid sequence, which is at least 95% identical to SEQ ID NO: 8; e) a nucleotide sequence which is at least 95% identical to SEQ ID NO: 7; or f) a nucleotide sequence according to c) or d) wherein the protein comprises a Glycine (G) on position 372 of SEQ ID NO: 8, or on a position corresponding thereto.
- Claim: 12. A marker for identifying a cucumber plant comprising a modified ABCB9 gene, wherein the marker detects a modified nucleotide on position 1282 as compared to the wild type ABCB9 gene nucleotide sequence of SEQ ID NO: 1.
- Claim: 13. The marker of claim 12, wherein the marker detects an Adenine (A) on position 1282 of SEQ ID NO: 3.
- Claim: 14. The marker of claim 13, comprising SEQ ID NO: 11.
- Claim: 15. A marker for identifying a cucumber plant comprising a modified EF1-α gene, wherein the marker detects a modified nucleotide on position 1115 as compared to the wild type EF1-α gene nucleotide sequence of SEQ ID NO: 5.
- Claim: 16. The marker of claim 15, wherein the marker detects a Guanine (G) on position 1115 of SEQ ID NO: 7.
- Claim: 17. The marker of claim 16, comprising SEQ ID NO: 12.
- Claim: 18. A method of identifying a cucumber plant that comprises a modified ABCB9 gene and/or a modified EF1-α gene, and in particular for identifying a plant that is resistant to CMV, comprising detecting the plant with a marker that detects a modified nucleotide on position 1282 as compared to the wild type ABCB9 gene nucleotide sequence of SEQ ID NO: 1 or a marker that detects a modified nucleotide on position 1115 as compared to the wild type EF1-α gene nucleotide sequence of SEQ ID NO: 5.
- Claim: 19. A method for producing a plant that is CMV resistant by introducing the modified ABCB9 gene of claim 1 and optionally introducing a modified EF1-α gene encoding a protein conferring resistance to CMV in a cucumber plant, in which the protein is expressed, characterized in that the gene comprises a nucleotide sequence of a) a nucleotide sequence which encodes a protein comprising SEQ ID NO: 8; b) a nucleotide sequence comprising SEQ ID NO: 7; c) a nucleotide sequence encoding a protein derived by substitution, deletion and/or addition of one or more amino acids of the protein comprising SEQ ID NO: 8, d) a nucleotide sequence that encodes a protein comprising an amino acid sequence, which is at least 95% identical to SEQ ID NO: 8; e) a nucleotide sequence which is at least 95% identical to SEQ ID NO: 7; or f) a nucleotide sequence according to c) or d) wherein the protein comprises a Glycine (G) on position 372 of SEQ ID NO: 8, or on a position corresponding thereto into the genome of a plant.
- Claim: 20. A method for selecting a CMV resistant plant, comprising a) identifying the presence of the modified ABCB9 gene of claim 1 and/or the presence of the modified EF-1 α gene of claim 3, b) selecting a plant that comprises at least one of said modified genes; c) optionally testing the selected plant for CMV resistance, and d) selecting the plant if it shows CMV resistance as a CMV resistant plant.
- Claim: 21. The method of claim 20, wherein the identification is performed with a marker for identifying a cucumber plant comprising a modified ABCB9 gene, wherein the marker detects a modified nucleotide on position 1282 as compared to the wild type ABCB9 gene nucleotide sequence of SEQ ID NO: 1 or the marker detects a modified nucleotide on position 1115 as compared to the wild type EF1-α gene nucleotide sequence of SEQ ID NO: 5.
- Claim: 22. A method for producing a plant that comprises the modified ABCB9 gene of claim 1, said method comprising: a) crossing a first parent plant comprising the modified ABCB9 gene, of claim 1, with a second parent plant, to obtain an F1 population, b) optionally performing one or more rounds of selfing and/or crossing with a plant from said F1 population to obtain a further generation population, c) selecting a plant that comprises the modified ABCB9 gene.
- Claim: 23. A method for producing a plant that comprises the modified EF1-α gene of claim 3, said method comprising: a) crossing a first parent plant comprising the modified EF1-α gene, with a second parent plant, to obtain an F1 population, b) optionally performing one or more rounds of selfing and/or crossing with a plant from said F1 population to obtain a further generation population, c) selecting a plant that comprises the modified EF1-α gene.
- Claim: 24. The method of claim 22, further comprising the steps of: d) testing the selected plant for showing CMV resistance; e) selecting the plant if it is CMV resistant.
- Claim: 25. The method of claim 23, further comprising the steps of: d) testing the selected plant for showing CMV resistance; e) selecting the plant if it is CMV resistant.
- Claim: 26. The method of claim 22, wherein the second parent plant also comprises a modified ABCB9 gene of claim 1.
- Claim: 27. The method of claim 23, wherein the second parent plant also comprises the modified EF1-α gene of claim 3.
- Claim: 28. The method of claim 22, wherein the first and/or the second parent plant also comprises the modified EF1-α gene of claim 3.
- Claim: 29. A method for selecting a plant that comprises the modified ABCB9 gene of claim 1 said method comprising; a) assaying nucleic acids of a plant for the presence of the modified ABCB9 gene of claim 1; b) identifying a plant that comprises the modified ABCB9 gene, and selecting said plant.
- Claim: 30. A method for selecting a plant that comprises the modified EF1-α gene, said method comprising; a) assaying nucleic acids of a plant for the presence of the modified EF1-α gene of claim 3, b) identifying a plant that comprises the modified EF1-α gene, and selecting said plant.
- Current International Class: 12; 12; 07
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