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Water soluble polymeric dyes and polymeric tandem dyes are provided. The polymeric dyes include a water solvated light harvesting multichromophore having a conjugated segment of aryl or heteroaryl co-monomers including branched non-ionic water soluble groups (WSG) comprising two or more water soluble polymers. In some cases, the branched non-ionic water soluble groups (WSG) of the present disclosure are capable of imparting solubility in water in excess of 50 mg/mL to the multichromophore. The polymeric tandem dyes further include a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Also provided are aggregation-resistant labelled specific binding members that include the subject water soluble polymeric dyes. Methods of evaluating a sample for the presence of a target analyte and methods of labelling a target molecule in which the subject polymeric dyes find use are also provided. Systems and kits for practicing the subject methods are also provided.

Titel:	Water-Soluble Polymeric Dyes
Link:	View record in USPTO Patent Applications (Volltext)
Veröffentlichung:	2023
Medientyp:	Patent
Sonstiges:	Nachgewiesen in: USPTO Patent Applications Sprachen: English Document Number: 20230116275 Publication Date: April 13, 2023 Appl. No: 17/956044 Application Filed: September 29, 2022 Claim: 1-79. (canceled) Claim: 80. A water soluble light harvesting multichromophore comprising a conjugated segment of repeating units, wherein at least one repeating unit is substituted with a branched non-ionic water soluble group (WSG) comprising two or more water soluble polymers. Claim: 81. The water soluble light harvesting multichromophore according to claim 80, wherein each water soluble polymer of each WSG comprises 6-50 monomeric units. Claim: 82. The water soluble light harvesting multichromophore according to claim 80, wherein the branched non-ionic water soluble group is capable of imparting solubility in water in excess of 50 mg/mL to the multichromophore. Claim: 83. The water soluble light harvesting multichromophore according to claim 80, wherein the branched non-ionic WSG has one of the following formulae: [chemical expression included] wherein: each B1 and B2 are independently a branching group; each W1 is independently a water soluble polymer comprising 6-24 monomeric units; T3 is an optional linker to the fused 6-5-6 tricyclic co-monomer; and each p and q are independently 0 or 1, wherein if present, each T1 and each T2 are independently a linker. Claim: 84. The water soluble light harvesting multichromophore according to claim 83, wherein: B1 is selected from CH, N, C(═O)N, SO2N, a tri-substituted aryl group, a tetra-substituted aryl group, and a tri-substituted heteroaryl group; each p is 0 or 1, wherein if present, each T1 is selected from —(CH2)n—O—, —(CH2)n— and —O—, wherein n is from 1 to 6; and T2 is a C1-C6-alkyl linker wherein one or more atoms are optionally substituted with a heteroatom. Claim: 85. The water soluble light harvesting multichromophore according to claim 80, wherein the branched non-ionic WSG is selected from one of the following structures: [chemical expression included] [chemical expression included] [chemical expression included] wherein: T5 is an optional linker to the fused 6-5-6 tricyclic co-monomer; T6 is a linker; each s is an integer from 6 to 24; and each R11 is independently hydrogen, an alkyl or a substituted alkyl. Claim: 86. The water soluble light harvesting multichromophore according to claim 80, wherein the conjugated segment of repeating units comprises fused cyclic repeat units. Claim: 87. The water soluble light harvesting multichromophore according to claim 80, wherein the conjugated segment of repeating units comprises aryl or heteroaryl repeat units. Claim: 88. The water soluble light harvesting multichromophore according to claim 80, wherein the multichromophore is linked to a specific binding member. Claim: 89. The water soluble light harvesting multichromophore according to claim 88, wherein the specific binding member comprises an antibody or binding fragment thereof. Claim: 90. A polymeric tandem dye comprising: a conjugated segment of repeating units, wherein at least one repeating unit is substituted with a branched non-ionic water soluble group (WSG) comprising two or more water soluble polymers; and a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith. Claim: 91. The tandem dye according to claim 90, wherein each water soluble polymer of each WSG comprises 6-50 monomeric units. Claim: 92. The tandem dye according to claim 90, wherein the branched non-ionic water soluble group is capable of imparting solubility in water in excess of 50 mg/mL to the multichromophore. Claim: 93. The tandem dye according to claim 90, wherein the branched non-ionic WSG has one of the following formulae: [chemical expression included] wherein: each B1 and B2 are independently a branching group; each W1 is independently a water soluble polymer comprising 6-24 monomeric units; T3 is an optional linker to the fused 6-5-6 tricyclic co-monomer; and each p and q are independently 0 or 1, wherein if present, each T1 and each T2 are independently a linker. Claim: 94. The tandem dye according to claim 93, wherein: B1 is selected from CH, N, C(═O)N, SO2N, a tri-substituted aryl group, a tetra-substituted aryl group, and a tri-substituted heteroaryl group; each p is 0 or 1, wherein if present, each T1 is selected from —(CH2)n—O—, —(CH2)n— and —O—, wherein n is from 1 to 6; and T2 is a C1-C6-alkyl linker wherein one or more atoms are optionally substituted with a heteroatom. Claim: 95. The tandem dye according to claim 90, wherein the branched non-ionic WSG is selected from one of the following structures: [chemical expression included] [chemical expression included] [chemical expression included] wherein: T5 is an optional linker to the fused 6-5-6 tricyclic co-monomer; T6 is a linker; each s is an integer from 6 to 24; and each R11 is independently hydrogen, an alkyl or a substituted alkyl. Claim: 96. The tandem dye according to claim 90, wherein the conjugated segment of repeating units comprises fused cyclic repeat units. Claim: 97. The tandem dye according to claim 90, wherein the conjugated segment of repeating units comprises aryl or heteroaryl repeat units. Claim: 98. The tandem dye according to claim 90, wherein the multichromophore is linked to a specific binding member. Claim: 99. The tandem dye according to claim 98, wherein the specific binding member comprises an antibody or binding fragment thereof. Claim: 100. The polymeric tandem dye according to claim 90, wherein the signaling chromophore is a fluorophore. Current International Class: 09; 01

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Water-Soluble Polymeric Dyes