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- Nachgewiesen in: USPTO Patent Grants
- Sprachen: English
- Patent Number: 10815,284
- Publication Date: October 27, 2020
- Appl. No: 16/336990
- Application Filed: August 31, 2017
- Assignees: NEXGEN BIOTECHNOLOGIES, INC. (Seoul, KR), Lee, Sun Kyo (Gyeonggi-Do, KR)
- Claim: 1. A fusion protein consisting of the amino acid sequence of SEQ ID NO: 5 or SEQ ID NO: 6.
- Claim: 2. A gene encoding the fusion protein of claim 1 .
- Claim: 3. The gene according to claim 2 , wherein the gene consists of any one of the nucleotide sequences of SEQ ID NOS: 1 or 2.
- Claim: 4. A recombinant vector comprising the gene of claim 2 .
- Claim: 5. A host cell transformed with the recombinant vector of claim 4 .
- Claim: 6. A method for producing a fusion protein in a host cell, the method comprising transforming the host cell with the recombinant vector of claim 4 to overexpress the gene encoding the fusion protein.
- Claim: 7. The method according to claim 6 , wherein the host cell is E. coli.
- Claim: 8. A cosmetic composition comprising the fusion protein of claim 1 , and at least one selected from the group consisting of a lipid material, an organic solvent, a dissolution agent, a condensation agent, a gelling agent, a softening agent, an anti-oxidant, a suspension agent, a stabilizer, a foaming agent, an aroma, a surface active agent, water, an ionic or non-ionic emulsifier, a filler, a metal ion sequestering agent, a chelating agent, a preservative, vitamin, a blocking agent, a moisturizing agent, essential oil, a dye, a pigment, a hydrophilic activating agent, a lipophilic activating agent, a common auxiliary agent, and a carrier.
- Claim: 9. The fusion protein of claim 1 , wherein the amino acid sequence is SEQ ID NO: 5.
- Claim: 10. The fusion protein of claim 1 , wherein the amino acid sequence is SEQ ID NO: 6.
- Claim: 11. A method for proliferating skin cells, the method comprising applying a composition comprising the fusion protein of claim 1 to the skin.
- Claim: 12. A method for improving skin wrinkle, the method comprising applying a composition comprising the fusion protein of claim 1 to the skin.
- Patent References Cited: 9321821 April 2016 Lee ; 10125181 November 2018 Lee ; 10266817 April 2019 Lee ; 10441632 October 2019 Lee ; 2006/0008440 January 2006 Blatt et al. ; 2016/0207962 July 2016 Ba et al. ; 63-3790 January 1988 ; WO 90/13571 November 1990 ; 2009-203184 September 2009 ; 10-1175803 August 2012 ; 10-1482187 January 2015 ; 10-2015-0056022 May 2015 ; 10-1636846 July 2016 ; 10-1636851 July 2016 ; 10-1661912 October 2016 ; 10-1678392 November 2016 ; WO 2014202089 December 2014
- Other References: Machine translation of KR 10-1636851 (published Jul. 6, 2016). cited by examiner ; Machine translation of KR 10-1636846 (published Jul. 7, 2016). cited by examiner ; Machine translation of KR 10-1482187 (published Jan. 14, 2015). cited by examiner ; Machine translation of KR 10-1661912 (published Oct. 4, 2016). cited by examiner ; Chao et al., Protein Science (1994), 3:1760-1769. cited by examiner ; Ng et al., Journal of Biological Chemistry, vol. 261, No. 33, Issue of Nov. 25, pp. 15690-15695,1986. cited by examiner ; International Search Report for PCT/KR2017/009514 dated Dec. 11, 2017. cited by applicant ; Zheng, Xueming et al., “Expression and Purification of Human Epidermal Growth Factor (hEGF) Fused with GB I”, Biotechnology & Biotechnological Equipment, vol. 30, No. 4, Apr. 2016. cited by applicant ; Li, Jia-Je et al., “Development and Characterization of the Recombinant Human VEGF-EGF Dual-targeting Fusion Protein as a Drug Delivery System”, Bioconjugate Chemistry, vol. 26, No. 12, 2015. cited by applicant ; Hanahan, D., “Studies on Transformation of Escherichia coli with Plasmids”, Mol. Biol., 166, 557-580, Jan. 1983. cited by applicant ; Carpenter G et al. “Epidermal Growth Factor” The Journal of Biological Chemistry vol. 265, 1990. cited by applicant ; Fallon et al., “Epidermal Growth Factor Immunoreactive Material in the Central Nervous System: Location and Development” Science 224 (4653): 1107-1109, 1984. cited by applicant ; Office action dated Mar. 10, 2020 from Japan Intellectual Property Office in a counterpart Japanese Patent Application No. 2019-517042. cited by applicant ; Li-Ming et al.,“Thermal stability Properties of an antifreeze protein from the desert beetle Microdera punctipennis”, Cryobiology, vol. 60, pp. 192-197, 2010. cited by applicant ; Nancy F.L. Ng et al., “Structure of an Antifreeze Polypeptide from the Sea Raven” J. Biol. Chemistry., vol. 267, No. 23, pp. 16069-16075, 1992. cited by applicant
- Primary Examiner: Allen, Marianne P
- Attorney, Agent or Firm: The PL Law Group, PLLC
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