Sonstiges: |
- Nachgewiesen in: USPTO Patent Grants
- Sprachen: English
- Patent Number: 11499,053
- Publication Date: November 15, 2022
- Appl. No: 17/316508
- Application Filed: May 10, 2021
- Assignees: BECTON, DICKINSON AND COMPANY (Franklin Lakes, NJ, US)
- Claim: 1. A flow cytometric system, comprising: a flow cytometer comprising a flow path; a composition in the flow path, wherein the composition comprises: a sample; and an aggregation-resistant labelled specific binding member comprising: a water soluble light harvesting multichromophore comprising a conjugated segment having the structure of formula (I): [chemical expression included] wherein: F 1 is a fused tricyclic co-monomer substituted with a water soluble group (WSG); M 1 is an aryl or heteroaryl co-monomer; n is an integer from 1 to 100,000; and * denotes a site for covalent attachment to the unsaturated backbone of a conjugated polymer or an end group; wherein at least one of F 1 and M 1 is substituted with a branched non-ionic water soluble group (WSG) comprising two or more water soluble polymers each having 6-50 monomeric units, wherein the branched non-ionic water soluble group has one of the following formulae: [chemical expression included] wherein: each B 1 and B 2 are independently a branching group selected from the group consisting of CH, N, C(═O)N, SO 2 N, a tri-substituted aryl group, a tetra-substituted aryl group, and a tri-substituted heteroaryl group; each W 1 is independently a water soluble polymer comprising 6-24 monomeric units; T 3 is an optional linker to the fused 6-5-6 tricyclic co-monomer; and each p and q are independently 0 or 1, wherein if present, each T 1 and each T 2 are independently a linker, wherein each T 1 is independently selected from the group consisting of (CH 2) n —O—, —O—(CH 2) n —, —(CH 2) n —, and —O—; and a specific binding member that specifically binds a target analyte and is covalently linked to the multichromophore.
- Claim: 2. The system according to claim 1 , wherein the labelled specific binding member further comprises a signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith.
- Claim: 3. The system according to claim 1 , wherein the composition further comprises a second specific binding member that is support bound and specifically binds the target analyte.
- Claim: 4. The system according to claim 3 , wherein the support comprises a magnetic particle.
- Claim: 5. The system according to claim 1 , wherein the sample comprises a cell.
- Claim: 6. The system according to claim 5 , wherein the target analyte is a cell surface marker of the cell.
- Claim: 7. The system according to claim 6 , wherein the cell surface marker is selected from the group consisting of a cell receptor and a cell surface antigen.
- Claim: 8. A kit comprising: a water soluble light harvesting multichromophore comprising a conjugated segment having the structure of formula (I): [chemical expression included] wherein: F 1 is a fused tricyclic co-monomer substituted with a water soluble group (WSG); M 1 is an aryl or heteroaryl co-monomer; n is an integer from 1 to 100,000; and * denotes a site for covalent attachment to the unsaturated backbone of a conjugated polymer or an end group; wherein at least one of F 1 and M 1 is substituted with a branched non-ionic water soluble group (WSG) comprising two or more water soluble polymers each having 6-50 monomeric units, wherein the branched non-ionic water soluble group has one of the following formulae: [chemical expression included] wherein: each B 1 and B 2 are independently a branching group selected from the group consisting of CH, N, C(═O)N, SO 2 N, a tri-substituted aryl group, a tetra-substituted aryl group, and a tri-substituted heteroaryl group; each W 1 is independently a water soluble polymer comprising 6-24 monomeric units; T 3 is an optional linker to the fused 6-5-6 tricyclic co-monomer; and each p and q are independently 0 or 1, wherein if present, each T 1 and each T 2 are independently a linker, wherein each T 1 is independently selected from the group consisting of (CH 2) n —O—, —O—(CH 2) n —, —(CH 2) n —, and —O—; and a container.
- Claim: 9. The kit according to claim 8 , further comprising one or more components selected from the group consisting of a polymeric tandem dye, a fluorophore, a specific binding member, a specific binding member conjugate, a cell, a support, a biocompatible aqueous elution buffer and instructions for use.
- Claim: 10. The kit according to claim 8 , wherein the multichromophore is covalently linked to a specific binding member.
- Claim: 11. The kit according to claim 10 , wherein the specific binding member is an antibody.
- Claim: 12. The kit according to claim 11 , wherein the specific binding member is an antibody fragment or binding derivative thereof.
- Claim: 13. The kit according to claim 12 , wherein the antibody fragment or binding derivative thereof is selected from the group consisting of a Fab fragment, a F(ab′) 2 fragment, a scFv, a diabody and a triabody.
- Claim: 14. The kit according to claim 8 , wherein multichromophore further comprises an acceptor signaling chromophore covalently linked to the multichromophore in energy-receiving proximity therewith.
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- Primary Examiner: Fang, Shane
- Attorney, Agent or Firm: Field, Bret E. ; Bozicevic, Field & Francis LLP
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