A short ORF-encoded transcriptional regulator
In: Proceedings of the National Academy of Sciences of the United States of America, Jg. 118 (2021-01-26), Heft 4
Online
academicJournal
- e2021943118
Recent technological advances have expanded the annotated protein coding content of mammalian genomes, as hundreds of previously unidentified, short open reading frame (ORF)-encoded peptides (SEPs) have now been found to be translated. Although several studies have identified important physiological roles for this emerging protein class, a general method to define their interactomes is lacking. Here, we demonstrate that genetic incorporation of the photo-crosslinking noncanonical amino acid AbK into SEP transgenes allows for the facile identification of SEP cellular interaction partners using affinity-based methods. From a survey of seven SEPs, we report the discovery of short ORF-encoded histone binding protein (SEHBP), a conserved microprotein that interacts with chromatin-associated proteins, localizes to discrete genomic loci, and induces a robust transcriptional program when overexpressed in human cells. This work affords a straightforward method to help define the physiological roles of SEPs and demonstrates its utility by identifying SEHBP as a short ORF-encoded transcription factor.
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A short ORF-encoded transcriptional regulator
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Autor/in / Beteiligte Person: | Koh, Minseob ; Ahmad, Insha ; Ko, Yeonjin ; Zhang, Yuxiang ; Martinez, Thomas F ; Diedrich, Jolene K ; Chu, Qian ; Moresco, James J ; Erb, Michael A ; Saghatelian, Alan ; Schultz, Peter G ; Bollong, Michael J |
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Zeitschrift: | Proceedings of the National Academy of Sciences of the United States of America, Jg. 118 (2021-01-26), Heft 4 |
Veröffentlichung: | eScholarship, University of California, 2021 |
Medientyp: | academicJournal |
Umfang: | e2021943118 |
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