Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain.
eScholarship, University of California, 2024
Online
academicJournal
Tissue-clearing and labeling techniques have revolutionized brain-wide imaging and analysis, yet their application to clinical formalin-fixed paraffin-embedded (FFPE) blocks remains challenging. We introduce HIF-Clear, a novel method for efficiently clearing and labeling centimeter-thick FFPE specimens using elevated temperature and concentrated detergents. HIF-Clear with multi-round immunolabeling reveals neuron circuitry regulating multiple neurotransmitter systems in a whole FFPE mouse brain and is able to be used as the evaluation of disease treatment efficiency. HIF-Clear also supports expansion microscopy and can be performed on a non-sectioned 15-year-old FFPE specimen, as well as a 3-month formalin-fixed mouse brain. Thus, HIF-Clear represents a feasible approach for researching archived FFPE specimens for future neuroscientific and 3D neuropathological analyses.
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Revealing intact neuronal circuitry in centimeter-sized formalin-fixed paraffin-embedded brain.
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Autor/in / Beteiligte Person: | Lin, Ya-Hui ; Wang, Li-Wen ; Chen, Yen-Hui ; Chan, Yi-Chieh ; Hu, Shang-Hsiu ; Wu, Sheng-Yan ; Chiang, Chi-Shiun ; Huang, Guan-Jie ; Yang, Shang-Da ; Chu, Shi-Wei ; Wang, Kuo-Chuan ; Lin, Chin-Hsien ; Huang, Pei-Hsin ; Chen, Bi-Chang ; Chu, Li-An ; Cheng, Hwai-Jong |
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Veröffentlichung: | eScholarship, University of California, 2024 |
Medientyp: | academicJournal |
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