Solid-phase extraction of nitro-PAH from aquatic samples and its separation by reverse-phase capillary liquid chromatography
In: Journal of the Brazilian Chemical Society, Jg. 18 (2007), Heft 5
Online
academicJournal
Zugriff:
In this work, an analytical methodology to extract 1-nitronaphthalene, 2-nitrofluorene, 9-nitroanthracene, 3-nitrofluoranthene, and 1-nitropyrene aquatic samples is proposed. Capillary liquid chromatography with on-column UV detection (µLC-UV) was employed to separate and quantitate all five nitrated polycyclic aromatic hydrocarbons (nitro-PAH) extracted from water samples. Two different extraction techniques were tested and compared: liquid-liquid extraction (LLE) and solid phase extraction (SPE) with two different sorbents, octadecylsilica (C18) and styrene divinylbenzene (XAD-2) particles. The elution of analytes was tested with both acetonitrile and methylene chloride. The best recovery results were obtained for SPE-C18 extraction with the methylene chloride with values ranging from 76 to 97% with RSD values smaller than 4%. The chromatographic conditions for analysis was carried out using a 250 × 0.3 mm i.d. packed capillary column with 5 µm C18 particles. The elution was isocratic with 65% acetonitrile in water (v/v) as mobile phase with a flow rate of 7 µL min-1. The detection limits due to on-column UV detection (ranging ~3-30 mmol L-1) are limiting for environmental applications, however, they are suited for toxicological studies.
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Solid-phase extraction of nitro-PAH from aquatic samples and its separation by reverse-phase capillary liquid chromatography
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Autor/in / Beteiligte Person: | Toledo, Marcelo ; Lanças, Fernando M. ; Carrilho, Emanuel |
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Zeitschrift: | Journal of the Brazilian Chemical Society, Jg. 18 (2007), Heft 5 |
Veröffentlichung: | Sociedade Brasileira de Química, 2007 |
Medientyp: | academicJournal |
ISSN: | 0103-5053 (print) |
DOI: | 10.1590/S0103-50532007000500019 |
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