STGC3 inhibits xenograft tumor growth of nasopharyngeal carcinoma cells by altering the expression of proteins associated with apoptosis
In: Genetics and Molecular Biology, Jg. 35 (2012), Heft 1
Online
academicJournal
Zugriff:
STGC3 is a potential tumor suppressor that inhibits the growth of the nasopharyngeal carcinoma cell line CNE2; the expression of this protein is reduced in nasopharyngeal carcinoma compared with normal nasopharyngeal tissue. In this study, we investigated the tumor-suppressing activity of STGC3 in nude mice injected subcutaneously with Tet/pTRE-STGC3/CNE2 cells. STGC3 expression was induced by the intraperitoneal injection of doxycycline (Dox). The volume mean of Tet/pTRE-STGC3/CNE2+Dox xenografts was smaller than that of Tet/pTRE/CNE2+Dox xenografts. In addition, Tet/pTRE-STGC3/CNE2+Dox xenografts showed an increase in the percentage of apoptotic cells, a decrease in Bcl-2 protein expression and an increase in Bax protein expression. A proteomic approach was used to assess the protein expression profile associated with STGC3-mediated apoptosis. Western blotting confirmed the differential up-regulation of prohibitin seen in proteomic analysis. These results indicate that overexpression of STGC3 inhibits xenograft growth in nude mice by enhancing apoptotic cell death through altered expression of apoptosis-related proteins such as Bcl-2, Bax and prohibitin. These data contribute to our understanding of the function of STGC3 in human nasopharyngeal carcinoma and provide new clues for investigating other STGC3-associated tumors.
Titel: |
STGC3 inhibits xenograft tumor growth of nasopharyngeal carcinoma cells by altering the expression of proteins associated with apoptosis
|
---|---|
Autor/in / Beteiligte Person: | Qiu, Qing-chao ; Hu, Bo ; He, Xiu-pei ; Luo, Qiao ; Tang, Guo-hua ; Long, Zhi-feng ; Chen, Zhu-chu ; He, Xiu-sheng |
Link: | |
Zeitschrift: | Genetics and Molecular Biology, Jg. 35 (2012), Heft 1 |
Veröffentlichung: | Sociedade Brasileira de Genética, 2012 |
Medientyp: | academicJournal |
ISSN: | 1415-4757 (print) |
DOI: | 10.1590/S1415-47572012005000009 |
Schlagwort: |
|
Sonstiges: |
|