Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater
In: Brazilian Archives of Biology and Technology, Jg. 60 (2017)
Online
academicJournal
Zugriff:
Trichosporon asahii and Rhodotorula mucilaginosa isolated from wastewater effluents were identified as chromium-resistant yeasts. Cr(VI) concentrations at 8 mM and 6 mM were inhibitory for R. mucilaginosa and T. asahii. Remarkably elevated GSH (69.88 ± 10.01) and GSSG (11.24 ± 0.96) was observed under metal stress in T. asahii as compared to R. mucilaginosa GSH (18.95 ± 3.19) and GSSG (3.7 ± 2.74) mM g-1 8 level. Statistical analysis revealed significantly higher GSH/GSSG ratio in both strains. NPSH (29.84 ± 0.54) level in T. asahii was much higher than in R. mucilaginosa (6.05 ± 0.24). Chromate reductase (ChR) was assayed and its activity was optimum at 50°C (pH 6) in T. asahii while R. mucilaginosa showed higher activity at 30°C (pH 7). Activity of both ChRs was enhanced in the presence of Mg, Na, Co and Ca but strongly inhibited by Hg cations. Cr(VI) uptake capabilities were ranged between 43-97% in R. mucilaginosa and 35-88% in T. asahii. One dimensional electrophoresis revealed enriched bands of cysteine rich metallothioneins suggesting some differential proteins could be overexpressed under Cr(VI) stress.
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Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater
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Autor/in / Beteiligte Person: | Elahi, Amina ; Rehman, Abdul |
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Zeitschrift: | Brazilian Archives of Biology and Technology, Jg. 60 (2017) |
Veröffentlichung: | Instituto de Tecnologia do Paraná - Tecpar, 2017 |
Medientyp: | academicJournal |
ISSN: | 1516-8913 (print) |
DOI: | 10.1590/1678-4324-2017160394 |
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