Osteoclastogenesis Modulation by Macrogeometrically Designed Superhydrophobic vs. Superhydrophilic Titanium Surfaces
2018
Online
unknown
Zugriff:
Objectives: The aim of this study was to evaluate the influence of implant macrodesign and surface hydrophilicity on osteoclast (OC) differentiation, activation, and survival in vitro. Methods: Macrogeometrically different titanium (Ti) disks were produced with a sandblasted, dual acid-etched surface, with or without an additional hydrophilic chemical modification (SAE-HD and SAE, respectively). Differences in the macrodesign comprised double and progressive squared threads with cutting chambers (HLX) versus double triangular threads (TMX). Four experimental groups were analysed: HLX/SAE-HD; HLX-SAE; TMX/SAE-HD; and TMX/SAE. Murine RAW 264.7 macrophages were seeded on the experimental disks, differentiated to OC by RANKL treatment and cultured for 5 days, or seeded on polystyrene positive [CCPC (+)] and negative controls [CCPC (–)], in triplicate. Tartrate-Resistant Acid Phosphatase (TRAP) activity and 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assays were conducted to evaluate osteoclast differentiation and viability, respectively. Gene expression levels of TRAP1, CTSK, MMP9, CALCR, ARG1, IL6, TNF and BAX were investigated and normalized against the expression of GAPDH and ACTB by real-time RT-PCR. Two independent experiments were performed for each analysis. General linear statistical models were applied for significant differences. Results: Both superhydrophilic surfaces, HLX/SAE-HD and TMX/SAE-HD, significantly induced less osteoclast differentiation (P<0.01), while TMX/SAE group generated significant higher TRAP activity in comparison with CCPC (+) (P<0.001). Cell viability was significantly different among all the experimental groups (P<0.001), significantly increased in TMX/SAE disks and negatively modulated in HLX/SAE-HD disks. The only difference in gene expression levels were related to osteoclast-related cytokines. Specifically, IL-6 and TNF were upregulated after 5 days of culture at the TMX/SAE-HD group compared with CCPC (+) (P<0.05). Conclusions: Chemically-modified superhydrophilic titanium surfaces negatively modulate RAW-OC formation and viability following 5 days of RANKL-induced osteoclastogenesis. Double triangular threads design, when presenting superhydrophobic surfaces, indicate a potential effect on osteoclast differentiation and cellular viability. Student Presenter This abstract is based on research that was funded entirely or partially by an outside source: CAPES (Full PhD Program, process no. 0975-14.1) and Neodent (process no. PAP-0303/16), Brazil. Disclosure Statement: The submitter must disclose the names of the organizations with which any author have a relationship, the nature of the relationship, and the clinical or research area involved. The following is submitted: NONE.
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Osteoclastogenesis Modulation by Macrogeometrically Designed Superhydrophobic vs. Superhydrophilic Titanium Surfaces
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Autor/in / Beteiligte Person: | Rezende de Jesus, Rainde Naiara ; Tsatsanis, Christos ; Zanetta-Barbosa, Darceny ; Stavropoulos, Andreas |
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Veröffentlichung: | 2018 |
Medientyp: | unknown |
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