Effects Of Dexamethasone In The Lens
In: Invest. Ophthalmol. Vis. Sci., Jg. 47 (2006), Heft 5, S. 4110-4110
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Zugriff:
Purpose: The aim of the study was to investigate effects of glucocorticoids in the lens. Methods: Lens epithelial cells (HLEC) were exposed to dexamethasone for 24 hours. Cells were assayed for changes in superoxide production using dihydroethidium (HET), for alterations in peroxide production using DCFH-DA or for GSH variations using monochlorobimane (MCB). Apoptosis was determined by Caspase-3 assay and by nuclear morphology of Hoechst stained cells. Mitochondria depolarisation was measured using the potential-sensitive colour JC-1. Morphology was examined by transmission electron microscopy (TEM). Results: Apoptosis were increased in HLEC exposed to 1, 10, 100 and 1000 {micro}M dexamethasone as revealed by nuclear morphology studies. Caspase-3 activity was increased at 100 and 1000 {micro}M dexamethasone. No effect on GSH, superoxide or peroxide production by dexamethasone was present. High concentrations of dexamethasone (1000 {micro}M) depolarised the mitochondria. TEM showed multilayering of cells, mitochondrial changes and accumulation of membrane delimited electron dense material. Conclusions: The mechanism underlying dexamethasone induced apoptosis and morphological changes in HLEC are probably not due to oxidative effects.
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Effects Of Dexamethasone In The Lens
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Autor/in / Beteiligte Person: | Petersen, Anne ; Zetterberg, Madeleine ; Carlsson, Therese ; Sjöstrand, Johan ; Karlsson, Jan-Olof |
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Zeitschrift: | Invest. Ophthalmol. Vis. Sci., Jg. 47 (2006), Heft 5, S. 4110-4110 |
Veröffentlichung: | 2006 |
Medientyp: | unknown |
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