Purinergic activation of a leak potassium current in freshly dissociated myocytes from mouse thoracic aorta.
In: Acta Physiologica, Jg. 195 (2009-02-01), Heft 2, S. 247-258
Online
academicJournal
Zugriff:
Aim: Exogenous ATP elicits a delayed calcium-independent K+ current on freshly isolated mouse thoracic aorta myocytes. We investigated the receptor, the intracellular pathway and the nature of this current. Methods: The patch-clamp technique was used to record ATP-elicited delayed K+ current in freshly dissociated myocytes. Results: ATP-elicited delayed K+ current was not inhibited by a ‘cocktail’ of K+ channel blockers (4-AP, TEA, apamin, charybdotoxin, glibenclamide). The amplitude of the delayed K+ current decreased after the reduction of extracellular pH from 7.4 to 6.5. These two characteristics suggest that this current could be carried by the TASK subfamily of ‘twin-pore potassium channels’ (K2P). Purinergic agonists including dATP, but not ADP, activated the delayed K+ current, indicating that P2Y11 is the likely receptor involved in its activation. The PKC activator phorbol ester 12,13-didecanoate stimulated this current. In addition, the PKC inhibitor Gö 6850 partially inhibited it. Real-time quantitative PCR showed that the genes encoding TASK-1 and TASK-2 are expressed. Conclusion: Our results indicate that blocker cocktail-insensitive delayed K+ current in freshly dissociated aortic myocytes is probably carried by the TASK subfamily of twin-pore channels. [ABSTRACT FROM AUTHOR]
Titel: |
Purinergic activation of a leak potassium current in freshly dissociated myocytes from mouse thoracic aorta.
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Autor/in / Beteiligte Person: | Hayoz, S. ; Bychkov, R. ; Serir, K. ; Docquier, M. ; Bény, J. L. |
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Zeitschrift: | Acta Physiologica, Jg. 195 (2009-02-01), Heft 2, S. 247-258 |
Veröffentlichung: | 2009 |
Medientyp: | academicJournal |
ISSN: | 1748-1708 (print) |
DOI: | 10.1111/j.1748-1716.2008.01884.x |
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